This ruby code builds upon the code S3 upload client for Ruby [dzone.com] by reading the account details and file details from XML files.

#!/usr/bin/env ruby
#file : rubys3file2upload.rb

require 'rubygems'
require 'aws/s3'
require 'rexml/document'
include REXML

class S3FileUpload

  def initialize(xml_accounts_file, xml_upload_file)
    initialize_account(xml_accounts_file)
    main(xml_upload_file)
  end

  def initialize_account(accounts_file)

    file = File.new(accounts_file)
    doc = Document.new(file)

    account = doc.root.elements['records/access']
    h = Hash.new
    h[:access_key_id] = account.elements['key_id'].text.to_s
    h[:secret_access_key] = account.elements['secret'].text.to_s
    AWS::S3::Base.establish_connection!(h)

  end

  def main(xml_upload_file)
    file = File.new(xml_upload_file)
    doc = Document.new(file)
puts doc
    doc.root.elements.each('records/file') do |f|
      local_file = f.elements['local'].text.to_s
      bucket = f.elements['bucket'].text.to_s
      mime_type = f.elements['mime_type'].text.to_s
      upload(local_file, mime_type, bucket)
    end
  end

  def upload(local_file, mime_type, bucket)

    base_name = File.basename(local_file)

    puts "Uploading #{local_file} as '#{base_name}' to '#{bucket}'"

    AWS::S3::S3Object.store(
      base_name,
      File.open(local_file),
      bucket,
      :content_type => mime_type,
      :access => :public_read
    )

    puts "Uploaded!"
  end

end

if __FILE__ == $0
  s3fu = S3FileUpload.new('s3accounts.xml', 's3files2upload.xml')
end

file: s3accounts.xml

<s3accounts>
  <summary/>
  <records>
    <access id="100"><name>REPLACE_ME</name><key_id>REPLACE_ME</key_id><secret>REPLACE_ME</secret></access>
  </records>
</s3accounts>

file: s3files2upload.xml

<s3files2upload>
  <summary/>
  <records>
    <file>
      <local>autocomplete.html</local>
      <bucket>t2000</bucket>
      <mime_type>text/html</mime_type>
    </file>
    <file>
      <local>autocomplete2.html</local>
      <bucket>t2000</bucket>
      <mime_type>text/html</mime_type>
    </file>
    <file>
      <local>autocomplete3.html</local>
      <bucket>t2000</bucket>
      <mime_type>text/html</mime_type>
    </file>
  </records>
</s3files2upload>

Note: The file given the correct permission could be read from http://t2000.s3.amazonaws.com/autocomplete.html

Reference: http://amazon.rubyforge.org/